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VRDye? 549 蛋白標(biāo)記試劑盒(高分子微量)
貨號(hào):928-54044
產(chǎn)品名稱: VRDye? 549 蛋白標(biāo)記試劑盒(高分子微量)
英文名:
CAS NO.:
分子式:
分子量:
產(chǎn)品品牌: Licor
產(chǎn)品規(guī)格: 1.5 mg
VRDye? 549 蛋白標(biāo)記試劑盒(高分子微量)
VRDye? 549 蛋白標(biāo)記試劑盒(高分子微量)
VRDye? 549 Protein Labeling Kit – High MW-Microscale, 1,5 mg
品牌:Licor 貨號(hào):928-54044
產(chǎn)地:USA (美國)
存儲(chǔ)條件:RT, 2-8 & -20
VRDye 蛋白標(biāo)記試劑盒經(jīng)過優(yōu)化,可標(biāo)記一抗或二抗,用于顯微鏡檢查、流式細(xì)胞術(shù)、免疫組織化學(xué) (IHC) 或其他需要熒光團(tuán)偶聯(lián)抗體的應(yīng)用。該試劑盒還可用于標(biāo)記分子量范圍為 50 kDa 至 200 kDa 的蛋白質(zhì)。試劑盒中包含的可見染料被 N-羥基琥珀酰亞胺 (NHS) 酯激活,這是標(biāo)記蛋白質(zhì)最常用的反應(yīng)基團(tuán)。 NHS 酯與伯胺反應(yīng),形成穩(wěn)定的共價(jià)鍵。該試劑盒針對標(biāo)記 100 μg 一抗或二抗 IgG 抗體進(jìn)行了優(yōu)化。
產(chǎn)品組份:
3 x 0.15 mg of VRDye 549 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 Zeba Desalting Spin Columns (store at 4 °C)
 
The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW-Microscale Quick Reference Guide.
VRDye? Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 100 μg of an IgG antibody.
 
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